E Reverse transcription, cDNA amplification, and NGS library preparation have been performed as in the typical Fall-seq process. f Sequencing was conducted working with an Illumina 75 bp NGS method. “Read one�?is made up of details about the cell barcode and UMI; “Browse two�?consists of information regarding the transcript. g https://www.quora.com/How-toxic-are-Grignard-Reagents/answer/Even-Sa?prompt_topic_bio=1